This service is based on the N-terminal Edman sequencing procedure. Classic Edman degradation chemistry remains the gold standard technique for N-terminal amino acid sequencing.

The analysis is applied to protein/peptide delivered in-solution or bound to a PVDF-membrane. Typically 6-20 residues can be determined by this process.

We provide
Automated Edman sequencing of the protein or peptide sample. Edman degradation is a cyclic procedure where the PITC reagent is coupled to the N-terminal amino group and cleaved off the protein. The PITC coupled residue is transferred to a flask, converted to a PTH-residue and the amino acid identified by HPLC chromatography. The reaction cycle is repeated and a sequence of N-terminal amino acids is determined.

You receive
You receive a detailed and easy to understand report (request sample report) in PDF format by e-mail containing:

• HPLC chromatograms
• Amino acid sequence after interpretation of the data. 

How to order

Order your FREE Pick 'n Post N-Terminal Sequencing kit today.  The kit contains all the material and information you need to ship samples in 10 minutes.

Ready to send samples right away? Download our Sample Submission Form & Guidelines. 
 

Additional service information

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